0 Пользователей и 1 Гость просматривают эту тему.
Having friends who share your passion has its advantages. Dan Bolser put me in contact with Justin Loe who together with Greg Magoon lead the FullGenomes company. FullGenomes has now teamed with a Chinese company, Novogene, located in Beijing, and are able to provide multiples of 1x whole genome sequencing for $65 each. Apparently, the co-creator of the well known genome assembler SOAPdenovo, Dr. Ruiqiang Li, is Novogene’s CEO.I wanted to give it a try and since I am short of cash these days, I am spending $130 for a 2x whole sequencing of my personal genome. The good thing about this deal is that the libraries are stored and can be used later if I have some more cash or I want to sequence the DNA with newer technologies.Justin has just told me that he has sent me 10 Biomatrica kits, which will be delivered promptly to my home address. It’s being a while since I have not felt the excitement of delving into the depths of our family genomes, but it seems that the time is ripe, the technology sufficiently accessible and the price affordable enough for the Corpas family to embark on the ultimate genome sequencing journey, the sequencing of our whole genomes.
То есть, скажем, если кто-то решит сделать с покрытием х50, полный геном обойдётся в 3250 долларов.Правильно понял?
Цитата: Mich Glitch от 01 Март 2015, 19:05:27То есть, скажем, если кто-то решит сделать с покрытием х50, полный геном обойдётся в 3250 долларов.Правильно понял?Из текста следует, что так:)Фаундеры пока молчат.
Hello,That is correct. $65/1x.Это правильно. $65/1x So1x = $655x = $325 [corrected]50x = $3250Note.30x is $1850
Цитата: warwick от 01 Март 2015, 22:34:02Hello,That is correct. $65/1x.Это правильно. $65/1x So1x = $655x = $325 [corrected]50x = $3250Note.30x is $1850Thank you for details.I'm just curious, which coverage is "reasonable" by you? I ask about price-quality ratio.x10?x20?x30?etc.
Could you estimate no-calls percentage for x30?
I'll look into it.
ChrY BAM file size: 0.72 Gb Reads (all): 12080535 Mapped reads: 12080535 (100.00%) Unmapped reads: 0 Length coverage: 13929068 bp (54.30%) Min depth coverage: 1X Max depth coverage: 7288X Mean depth coverage: 91.23X Median depth coverage: 60X No call: 11724498 bp
Цитата: warwick от 01 Март 2015, 22:55:52I'll look into it.Just to compare, here is the last results for one of my samples FTDNA (lab processing) + Yfull (interpretation):ЦитироватьChrY BAM file size: 0.72 Gb Reads (all): 12080535 Mapped reads: 12080535 (100.00%) Unmapped reads: 0 Length coverage: 13929068 bp (54.30%) Min depth coverage: 1X Max depth coverage: 7288X Mean depth coverage: 91.23X Median depth coverage: 60X No call: 11724498 bp
интересно по тесту " x1 " можно получить 460 STR маркеров после интерпретации?