TAT-C, M96, M89, M9, M45
Я не вчитывался в статью, а надо было. Пока не так и не понял момента, на M45 на остальные 2 образца типирование прошло успешно, или нет. То, что никто из лаборантов не N1c я понял
For ancient DNA, bones from 8 skeletons were studied, but in
only four cases was DNA successfully isolated. These four samples
were derived from three different well-documented Hungarian
excavations from 10th century cemeteries
***
whereas out of the four successfully typed
10th-century skeletal remains two (anc21 and anc28), possess
the mutation, while the samples anc4 and anc19 carry
the ancestral T allele.
***
Figure 3 denotes the multiplex amplification (markers
M9, M45) of ancient DNA from sample anc19. The presence
of the ancestralCallele of theM9polymorphism reinforced
the fact that this sample does not harbour themutant
Tat C allele, in accordance with standard Y-chromosome
phylogeny. Out of four Y-chromosomal DNA fragments,
containing the four basal markers (M96, M89, M9 and
M45), the two shorter fragments (106 bp and 121 bp long
specific PCR products) were successfully amplified, where
the signal reflecting the M45 marker was stronger. Such an
inverse relation between the efficiency of amplification and
the length of the amplified product is typical of DNA retrieved
from archaeological remains and results from damage
and degradation of the DNA (Handt et al., 1994). The
loci M89 and M96 could not be amplified, probably because
they are in the higher molecular weight range.
No one from the Archaeological Institute who has handled
the bones (excavator, anthropologist) has the Tat C
mutation, which strongly supports the authenticity of the
ancient DNA.
Тема: Re: Результаты исследований дДНК (обсуждение) (Прочитано 748467 раз)