Y CHROMOSOME SINGLE NUCLEOTIDE POLYMORPHISMS TYPING BY SNaPshot MINISEQUENCING

[Centurion]

Y CHROMOSOME SINGLE NUCLEOTIDE POLYMORPHISMS TYPING BY SNaPshot MINISEQUENCING // BALKAN JOURNAL OF MEDICAL GENETICS, 12 (2), 2009

Noveski et al.

ABSTRACT
Analysis of Y chromosome haplogroups, defined
by single nucleotide polymorphisms (SNPs),
is now a standard approach for study of the origin
of human populations and measurement of the variability
among them. It is also a new forensic tool,
because it may allow determination of the origin of
any male sample of interest. We have used a strategy
for rapid, simple and inexpensive Y chromosome
SNP typing of 343 male DNA samples, of which 211
were Macedonians, 111 Albanians and 21 Roma,
Serbs or Turks. Using multiplex polymerase chain
reaction (mPCR) and a SNaPshot multiplex kit for
single nucleotide extension reaction, 28 markers
were grouped into five multiplexes. Twenty different
Y haplogroups were found in these samples.
The most common Y haplogroups in Macedonians
were I2a-P37b (27.5%), E1b1b1a-M78 (15.6%),
R1a1-SRY1532 (14.2%) and R1b1-P25 (11.4%). In
the Albanians E1b1b1a-M78 accounted for 28.8%,
R1b1-P25 for 18.0%, J2b2-M241 for 13.5% and
R1a1-SRY1532 for 12.6%. We conclude that five
haplogroups (E1b1b1a-M78, I2a-P37b, J2b2-M241,
R1a1-SRY1532 and R1b1-P25) comprised 72.6%
of the Y chromosomes, this being characteristic of
the typical European Y chromosome gene pool.

http://www.bjmg.edu.mk/UploadedImages/pdf/BJMG_03,vol.12(2),2009.pdf