WANG Chi-zao1, WEN Shao-qing1, SHI Mei-sen2, YU Xue-er1, WAN Xue-jiao3, PAN Yi-ling3, ZHANG
Yun-fei3, LI Hui1, TAN Jing-ze1
(1. MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200438, China; 2. Key Laboratory of Evidence Law and Forensic Science, Ministry of Education, China
University of Political Science and Law, Beijing 100088, China; 3. Natural History Research Center, Shanghai Science and Technology Museum, Shanghai 200127, China)
Abstract: Objective To identify the Y-chromosomal genetic types for the soldier’s remains from Huaihai Campaign, and to offer a clue for search of their paternal relatives. Methods DNA of the remains
were extracted by the ancient DNA extraction method. Yfiler kit was used for the multiplex amplification of 17 Y-STR loci. The haplogroups of the samples were speculated. Detailed genotyping of the selected Y-SNP was performed based on the latest Y-chromosome phylogenetic tree. Haplotype-sharing
analysis was done based on the data of Y-SNP and Y-STR, the closest modern individual information
to the genetic relationship of remains was gained. Results A total of 8 Y-STR haplotypes were observed
on 17 Y-STR loci of 8 male individuals. Furthermore, 6 Y -SNP haplogroups were identified, which
were O2a1-M95+, O1a1-P203+, O3*-M122+/M234-, D1-M15+, C3*-ST and R1a1-M17+. Conclusion
Identification of Y-chromosomal genetic types for the soldier’s remains from Huaihai Campaign shows a
reference value on inferring the geographical origins of old materials.
Keywords: forensic genetics; Y chromosome; military personnel; personal identification; Huaihai Campaign