АвторТема: Y-chromosomal SNPs in Finno-Ugric-speaking populations analyzed by minisequencin  (Прочитано 2645 раз)

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Y-chromosomal SNPs in Finno-Ugric-speaking populations analyzed by minisequencing on microarrays // Genome Res. 2001 11: 471-482

Raitio et al

Abstract

An increasing number of single nucleotide polymorphisms (SNPs) on the Y chromosome are being identified.
To utilize the full potential of the SNP markers in population genetic studies, new genotyping methods with
high throughput are required. We describe a microarray system based on the minisequencing single nucleotide
primer extension principle for multiplex genotyping of Y-chromosomal SNP markers. The system was applied
for screening a panel of 25 Y-chromosomal SNPs in a unique collection of samples representing five
Finno–Ugric populations. The specific minisequencing reaction provides 5-fold to infinite discrimination between
the Y-chromosomal genotypes, and the microarray format of the system allows parallel and simultaneous
analysis of large numbers of SNPs and samples. In addition to the SNP markers, five Y-chromosomal
microsatellite loci were typed. Altogether 10,000 genotypes were generated to assess the genetic diversity in
these population samples. Six of the 25 SNP markers (M9, Tat, SRY10831, M17, M12, 92R7) were polymorphic in the
analyzed populations, yielding six distinct SNP haplotypes. The microsatellite data were used to study the
genetic structure of two major SNP haplotypes in the Finns and the Saami in more detail. We found that the
most common haplotypes are shared between the Finns and the Saami, and that the SNP haplotypes show
regional differences within the Finns and the Saami, which supports the hypothesis of two separate settlement
waves to Finland.

http://genome.cshlp.org/content/11/3/471.full.pdf+html

 

© 2007 Молекулярная Генеалогия (МолГен)

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