АвторТема: Allele frequencies of the five miniSTR loci D1S1656, D2S441, D10S1248, D12S391  (Прочитано 1995 раз)

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Allele frequencies and resulting statistical parameters of the five miniSTR loci D1S1656, D2S441, D10S1248, D12S391 and D22S1045 (“European recommended loci”) were obtained from a sample of 404 unrelated individuals from South Germany. No deviations from Hardy–Weinberg equilibrium were observed. Amplification was done simultaneously in a PCR-multiplex reaction with a maximum fragment size of less than 175 bp. Due to the small amplicon length this multiplex is particularly suitable for the analysis of degraded DNA samples.

Blood samples and buccal cell swabs were collected from 404 randomly selected, unrelated individuals living in Bavaria, South Germany, and typed in anonymized form. DNA was extracted using Chelex® 100 . All markers were amplified simultaneously using the primer sequences depicted in Table 1. Amplification parameters were as follows: hot start (12 min, 95 °C), 30 cycles (60 s, 93 °C; 60 s, 59 °C; 90 s, 72 °C), final step (45 min, 60 °C). Amplified products were separated using an ABI 3130 genetic analyzer (Applied Biosystems, Foster City, USA). Subsequent analysis was done using the GeneMapper Software, Vers. 3.2 (Applied Biosystems, Foster City, CA). Allele designations were made according to recommendations of the European DNA profiling group (EDNAP)  with the aid of allelic ladders based on the commercially available DNA control samples K562, 9947A (Promega, Madison, USA) and 007 (Applied Biosystems, Foster City, USA). The genotypes of theses samples are given in Table 1. Quality control is carried out by regular participation in the GEDNAP blind trials (http://www.gednap.de). The authors agree with the guidelines for publication of population data  A. Carracedo, J.M. Butler, L. Gusm?o, W. Parson, L. Roewer and P.M. Schneider, Publication of population data for forensic purposes, Forensic Sci. Int. Genet. 4 (2010), pp. 145–147.


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